Fig. 3

Comparison of vmat2 and th expression patterns. (A–H) Whole-mount in situ hybridization detecting vmat2 (A–D) and th (E–H) expression in 48 hpf (A, B, E, F) and 4 dpf (C, D, G, H) embryos. (A, C, E, G) Lateral views, (B, D, F, H) dorsal views, rostral to the left. Vmat2-reactive neurons in ventral diencephalon can be divided into anterior group of posterior tuberculum (PTa), posterior group of posterior tuberculum (PTp) and group of paraventricular organ (asterisk), and there is a gap (white arrow) between PTa and PTp; (H) catecholaminergic clusters in ventral diencephalon of 4 dpf embryos can be divided into several groups from anterior to posterior: population 1 (between ventral thalamus and posterior tuberculum), population 2 (anterior group of posterior tuberculum), population 3 (paraventricular organ), population 4 (posterior group of posterior tuberculum) and populations 5 and 6 (between posterior tuberculum and hypothalamus); there is also a gap (white arrow) between populations 2 and 4. AAN: arch-associated neurons; DC: diencephalic catecholaminergic cluster; DV: diencephalic vmat2-expression cluster; HyV: hypothalamic vmat2-expression cluster; LC: locus coeruleus; MC: medulla catecholaminergic cluster; PTa: anterior group of the posterior tubercular neurons; PTp: posterior group of the posterior tubercular neurons; PtC: pretectal catecholaminergic cluster; PtV: pretectal vmat2-expression cluster; Ra: raphe nuclei; Sym: sympathetic neurons.