Fig. 4
- ID
- ZDB-FIG-080303-8
- Publication
- Wen et al., 2008 - Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish
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Double immunofluorescence staining for detection of GFP and endogenous Th. Simultaneous immunofluorescence staining was generated using a polyclonal anti-GFP antibody (A, D, G) and a monoclonal anti-Th antibody (B, E, H) for 30 hpf (A–F) and 3 dpf (G–I) ETvmat2:GFP embryos. Composite photos were made from stacked confocal images, focusing on catecholaminergic neurons of the ventral diencephalon. Merged pictures of Th and GFP staining are shown in panels C and F. Higher magnification images of the posterior tubercular neurons (D–F) correspond to the framed region in panel C. |
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Stage Range: | Prim-15 to Protruding-mouth |
Reprinted from Developmental Biology, 314(1), Wen, L., Wei, W., Gu, W., Huang, P., Ren, X., Zhang, Z., Zhu, Z., Lin, S., and Zhang, B., Visualization of monoaminergic neurons and neurotoxicity of MPTP in live transgenic zebrafish, 84-92, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.