Molecular characterization of myogenesis corresponds with the location of new, small diameter fibers. A-C: Expression of molecular markers of myogenesis in 6.0 mm larval pectoral fins. A: Pax7 (Green) is expressed in cells superficial to the existing muscle masses (MF20, Cyan). A subset of Pax7 expressing cells coexpress Myogenin (Red), signifying myogenic differentiation. Insets signify boxed region and show a nucleus that expresses both Pax7 and Myogenin (arrowheads). B: The muscle regulatory factor myod is expressed by cells at the periphery of the muscle masses. C: Myogenin is expressed by cells at the periphery of the muscle masses. Scale bar = 25 μm.
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