FIGURE

Fig. 3

ID

ZDB-FIG-071213-12

Publication

Park et al., 2007 - An olig2 reporter gene marks oligodendrocyte precursors in the postembryonic spinal cord of zebrafish

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Fig. 3

olig2⁺ spinal cord cells divide at postembryonic stages. All images are single confocal optical sections of transverse sections of 30 dpf Tg(olig2:egfp) zebrafish spinal cords, dorsal up. A: Graph showing average number of BrdU⁺ spinal cord cells per transverse section at different embryonic and postembryonic stages. Data were compiled from 15-30 sections per timepoint. Bars represent standard error of the mean. B: 4 dpf Tg(olig2:egfp) transgenic larvae pulse-treated with BrdU and labeled with anti-BrdU antibody. Arrows indicate EGFP⁺ radial glia, which incorporated BrdU. C,D: Twenty- and 30-dpf Tg(olig2:egfp) transgenic fish treated with BrdU repeatedly. Many cells, including EGFP⁺ radial glia (arrows), bordering the central canal and medial septum incorporated BrdU. Some EGFP⁺ oligodendrocyte lineage cells were also BrdU⁺ (arrowheads). E: Double labeling with anti-Sox10 and anti-PCNA antibodies. Sox10⁺ PCNA⁺ cells were proliferative OPCs (arrowheads). Scale bar = 30 μM (B,C), 50 μM (D,E).

Expression Data

Genes:	pcna sox10
Fish:	vu12Tg
Anatomical Term:	oligodendrocyte
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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