PUBLICATION

An olig2 reporter gene marks oligodendrocyte precursors in the postembryonic spinal cord of zebrafish

Authors
Park, H.C., Shin, J., Roberts, R.K., and Appel, B.
ID
ZDB-PUB-071118-16
Date
2007
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   236(12): 3402-3407 (Journal)
Registered Authors
Appel, Bruce, Park, Hae-Chul, Shin, Jimann
Keywords
spinal cord, oligodendrocyte progenitor, radial glia, zebrafish transgenic, adult neural stem cell
MeSH Terms
  • Adult Stem Cells/cytology*
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Genes, Reporter
  • Green Fluorescent Proteins/genetics
  • Nerve Tissue Proteins/genetics*
  • Neuroglia/cytology
  • Oligodendroglia/cytology*
  • Recombinant Proteins/genetics
  • Spinal Cord/cytology*
  • Spinal Cord/growth & development
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish Proteins/genetics*
PubMed
17969181 Full text @ Dev. Dyn.
Abstract
Continuous production of new neurons and glia in adult mammals occurs within specialized proliferation zones of the forebrain. Neural cell proliferation and neurogenesis is more widespread in adult amphibians, reptiles, and fish but the identity of neural stem cell populations in these organisms has not been fully described. We investigated expression of a reporter gene driven by olig2 regulatory DNA at postembryonic stages in zebrafish. We show that olig2 expression marks a discrete population of spinal cord radial glia in larvae and adults that divide continuously. olig2(+) radial glia have hallmarks of stem cells and their divisions appear to be asymmetric, producing new oligodendrocytes but not neurons or astrocytes.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping