Co-depletion of keratins phenocopies the FoxH1 morphant phenotype. (A–D) Morphological similarity of FoxH1 morphants and keratin morphants. Wild type embryos were injected with 8 ng of FoxH1 MO1, 16 ng of control MO, or 16 ng of a mixture containing 8 ng, 2 ng, 2 ng, 2 ng and 2 ng of MOs respectively targeting the translation of Cyt1, Cyt2, Keratin 4, Keratin 8 and Keratin 18. Un-injected (Uninj.) embryos are also shown to illustrate the moderate delays associated with a high dose (16 ng) of control MO. Injected embryos were kept in their chorions and placed into conical wells formed in 2% agarose, then simultaneously time lapse documented. Primes (′) indicate different time points for the same embryos. Most views are lateral, however embryos were free to rotate during the time lapse and panel A′ is closer to a frontal or ventral view. (E–P) Co-depletion of keratins phenocopies marker expression in FoxH1 morphants. Wild type embryos were injected with 8 ng of FoxH1 MO1, 16 ng of control MO, or 16 ng of the keratin MO mixture mentioned above and fixed at 8 hpf, when control embryos reached 70% epiboly, for in situ analysis. Panels E–H are dorsal views, and panels I–P, for which the dorsal side could not be determined, are randomly oriented side views. Abbreviations: FH1, FoxH1; ctrl, control.
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