Fig. S4
Expression of gata1 and pu.1 in Class II CA alk8 mRNA-Injected Embryos Expression of gata1 and pu.1 was increased in the PLPM (as expected in ventralized embryos); pu.1 expression in the ALPM was expanded but gata1 was absent from the ALPM. gata1 expression was normal in WT alk8 mRNA-injected embryos (A) but was increased posteriorly in ventralized CA alk8 mRNA-injected embryos (B) (n = 37/54) at 8?10 somites. In embryos analyzed for expanded ALPM pu.1 expression (Figures 2E and 2G), posterior pu.1 expression was normal in WT alk8 mRNA-injected embryos with only weak expression posteriorly (C), but was increased in CA alk8 mRNA-injected embryos (D). (A) and (B) show gata1 expression, and (C) and (D) show pu.1 expression. |