ZFIN ID: ZDB-FIG-070928-2
Hirata et al., 2007 - Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development (Cambridge, England)   134(15):2771-2781 Full text @ Development
Observed In:
Stage: Long-pec

Fig. 3 Ca2+ transients are smaller in ryr mutant fast muscles. (A) Schematic summary of the experimental procedure. Calcium Green-1 dextran was injected into one cell of 8- to 16-cell-stage embryos. Embryos (48 hpf) were pinned on a dish and given mechanosensory stimulation by a puff of liquid to the tail. The relative level of Calcium Green-1 fluorescence ({Delta}F/F) in muscle fibers was assayed by line-scanning with a confocal microscopy. (B) The transient increase in relative fluorescence represents the transient increase of Ca2+ in the fast muscle following stimulation (arrow) of a wild-type embryo (black) and that of an ryr mutant (red). (C) The transient increase of Ca2+ in slow muscle is unperturbed in ryr mutants (red) compared to wild-type (black).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ryr1bmi340/mi340 standard conditions Long-pec ryanodine-sensitive calcium-release channel activity decreased occurrence, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development