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ZIRC
ZFIN ID: ZDB-FIG-070813-9
Hirata et al., 2007 - Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development (Cambridge, England)   134(15):2771-2781 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Adult

Fig. 6 ryr1b and ryr1a are expressed by fast and slow muscle, respectively. (A) Reverse transcriptase (RT)-PCR shows that both ryr1b and ryr1a are expressed at 1, 2 and 3 dpf, and in adults. RT-PCR with mRNA from deep muscles indicates that ryr1b but not ryr1a is expressed by adult fast muscles. (B-I) In situ hybridization with ryr1b (B-E) and ryr1a (F-I) probes. Wholemounts show that both genes are expressed by muscle at 24 (B,F) and 48 (D,H) hpf. Cross-sections show that ryr1b is expressed by deep, fast muscles (C,E), whereas ryr1a is expressed by superficial, slow muscles but not by fast muscles (G,I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ryr1a WT standard conditions Prim-5 Not Detected fast muscle cell ISH
Prim-5 slow muscle cell ISH
Prim-5 whole organism RTPCR
Long-pec Not Detected fast muscle cell ISH
Long-pec slow muscle cell ISH
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Adult Not Detected fast muscle cell RTPCR
Adult trunk RTPCR
ryr1b WT standard conditions Prim-5 fast muscle cell ISH
Prim-5 Not Detected slow muscle cell ISH
Prim-5 whole organism RTPCR
Long-pec fast muscle cell ISH
Long-pec Not Detected slow muscle cell ISH
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Adult fast muscle cell RTPCR
Adult trunk RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development