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ZFIN ID: ZDB-FIG-070813-7
Hirata et al., 2007 - Zebrafish relatively relaxed mutants have a ryanodine receptor defect, show slow swimming and provide a model of multi-minicore disease. Development (Cambridge, England)   134(15):2771-2781 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4 E-C-coupling components are dramatically decreased in ryr fast muscles. (A-H,K-R) The distribution of RyRs and DHPRs were assayed in fast and slow muscles at 48 hpf with anti-RyR (A,B,K,L), anti-DHPRα1 (C,D,M,N), anti-DHPRß (E,F,O,P) and anti-α2 (G,H,Q,R). Wild-type fast muscles express RyR (A), DHPRα1 (C), DHPRß (E) and DHPRα2 (G) in a striated pattern that presumably corresponds to the t-tubule-SR junctions, whereas clustering is not observed in mutant fast muscles (B,D,F,H). Wild-type slow muscles express RyR (K), DHPRα1 (M), DHPRß (O) and DHPRα2 (Q) in a striated pattern, as in wild-type fast muscles. ryr mutant slow muscles also express RyR (L), DHPRα1 (N), DHPRß (P) and DHPRα2 (R) in a striated pattern. (I,J,S,T) RyR (green) and DHPRα2 (purple) are colocalized in wild-type fast (I) and slow (S) muscle and in mutant slow muscle (T) but not in ryr mutant fast muscle (J). Insets show a higher magnification of muscle fibers. (U-X) Electron micrographs of t-tubule-SR junctions in muscles at 48 hpf. Putative RyR-DHPR aggregates are visible as dense particles between t-tubule and SR membranes in wild-type fast muscle (U) but not in ryr mutant fast muscle (V). The RyR-DHPR aggregates are present in both wild-type (W) and mutant (X) slow muscles. SR, sarcoplasmic reticulum.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ryr1bmi340/mi340 standard conditions Long-pec voltage-gated calcium channel activity decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development