ZFIN ID: ZDB-FIG-070914-62
Chopra et al., 2007 - Molecular cloning and analysis of zebrafish voltage-gated sodium channel beta subunit genes: Implications for the evolution of electrical signaling in vertebrates. BMC Evolutionary Biology   7(1):113 Full text @ BMC Evol. Biol.
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 5 Zebrafish sodium channel β1-4 subunit genes and novel splice variants are differentially expressed in excitable tissues. Total RNA was isolated from wild-type adult zebrafish tissues. RT-PCR with gene and splice variant-specific primers was used to detect expression (see Table 5 for primer sequences and amplicon details). Atr = atrium, Ven = ventricle, Skm = skeletal muscle, Brn = Brain, Eye = eye/optic nerve, Liv = liver, Gil = gill. + = enzyme added to reverse transcription step, - = no reverse transcriptase enzyme (negative control). Zebrafish β-actin was amplified from each template as a positive control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
scn1ba AB/TU standard conditions Adult atrium RTPCR
Adult Not Detected atrium RTPCR
Adult Not Detected atrium RTPCR
Adult Not Detected atrium RTPCR
Adult brain RTPCR
Adult Not Detected brain RTPCR
Adult brain RTPCR
Adult brain RTPCR
Adult Not Detected cardiac ventricle RTPCR
Adult cardiac ventricle RTPCR
Adult Not Detected cardiac ventricle RTPCR
Adult Not Detected cardiac ventricle RTPCR
Adult eye RTPCR
Adult Not Detected eye RTPCR
Adult eye RTPCR
Adult eye RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected gill RTPCR
Adult gill RTPCR
Adult Not Detected liver RTPCR
Adult liver RTPCR
Adult Not Detected liver RTPCR
Adult Not Detected liver RTPCR
Adult musculature system RTPCR
Adult musculature system RTPCR
Adult Not Detected musculature system RTPCR
Adult Not Detected musculature system RTPCR
scn2b AB/TU standard conditions Adult atrium RTPCR
Adult atrium RTPCR
Adult Not Detected atrium RTPCR
Adult atrium RTPCR
Adult brain RTPCR
Adult brain RTPCR
Adult brain RTPCR
Adult brain RTPCR
Adult Not Detected cardiac ventricle RTPCR
Adult cardiac ventricle RTPCR
Adult cardiac ventricle RTPCR
Adult cardiac ventricle RTPCR
Adult eye RTPCR
Adult eye RTPCR
Adult eye RTPCR
Adult eye RTPCR
Adult Not Detected gill RTPCR
Adult gill RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected liver RTPCR
Adult Not Detected liver RTPCR
Adult Not Detected liver RTPCR
Adult liver RTPCR
Adult Not Detected musculature system RTPCR
Adult Not Detected musculature system RTPCR
Adult Not Detected musculature system RTPCR
Adult Not Detected musculature system RTPCR
scn3b AB/TU standard conditions Adult atrium RTPCR
Adult brain RTPCR
Adult cardiac ventricle RTPCR
Adult eye RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected liver RTPCR
Adult Not Detected musculature system RTPCR
scn4ba AB/TU standard conditions Adult atrium RTPCR
Adult brain RTPCR
Adult cardiac ventricle RTPCR
Adult eye RTPCR
Adult Not Detected gill RTPCR
Adult Not Detected liver RTPCR
Adult musculature system RTPCR
scn4bb AB/TU standard conditions Adult Not Detected atrium RTPCR
Adult brain RTPCR
Adult cardiac ventricle RTPCR
Adult eye RTPCR
Adult Not Detected gill RTPCR
Adult liver RTPCR
Adult Not Detected musculature system RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Evolutionary Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Evol. Biol.