FIGURE

Fig. 3

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ZDB-FIG-070822-52

Publication

Hinits et al., 2007 - Mrf4 (myf6) is dynamically expressed in differentiated zebrafish skeletal muscle

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Fig. 3

Mrf4 mRNA is expressed late in muscle terminal differentiation. In situ mRNA hybridisation for mrf4 (blue, A, C–K), eng2a (B) and mylz2 (K) and wholemount immunohistochemistry for MyHC (brown, B, F) in zebrafish embryos viewed in wholemount (E, G, H, K lateral view, dorsal to right) or flatmount (A–D, J, dorsal view), anterior to top, or transverse section (F, I; dorsal to top). (A) Mrf4 expression is missing in gli2a^ty119/ty119 embryos and reduced in heterozygotes gli2a^ty119/+ compared to wild type siblings. (B) At 15s, shha mutant embryos lack eng2a-expressing MPs, but have residual MyHC-expressing SSF precursors (black arrows), whereas sibling embryos show eng2a expression in MPs anterior to the three youngest somites (between red arrows) and MyHC in SSF precursors (black arrows). (C) shha mutants show no mrf4 MP expression prior to 16s, in contrast to their siblings. (D) Similarly, prdm1 mutants show faint mrf4 expression in adaxial cells but lack transient strong expression. (E) Mrf4 is widely expressed at 24hpf in wild type. Anteriorly, mrf4 expression fills the bulk of the somite chevron. Note transient dorsoventral midline expression in the most caudal newly formed somites (arrowhead). shha mutant lacks MP mrf4 staining in caudal somites (arrowhead), but retains strong expression in more anterior U-shaped somites (arrow). (F) Rostral section of a 21s shha embryo, identified by U-shape somites and lack of caudal MP mrf4 expression, showing that MyHC closely co-localises with mrf4 mRNA in differentiated SSF precursor fibres migrating towards the lateral surface of the somite. Eosin counterstain. (G and H) At 24 and 25s, both prdm1 (G) and gli2a (H) mutants and sibling show mrf4 expression in fast muscle cells in more rostral somites, whereas only the sibling shows caudal slow expression (arrowheads). (I) Gli2a mutant with no slow muscle (MPs or SSFs) expresses mrf4 in fast muscle cells. (J) Fgf8 morphants show no change in mrf4 expression at 15s. (K) Mylz2(fast myosin light chain 2) co-localises with mrf4 in fast fibres but not in slow fibres (arrowhead) in fgf8 mutants and siblings. In fgf8 mutants, mrf4 mRNA is reduced in parallel with differentiated fast muscle (insets), but is unchanged in slow.

Expression Data

Genes:	en2a myf6 mylpfa
Fish:	WT fgf8a^{ti282a/ti282a} gli2a^ty119/ty119 prdm1a^tp39/tp39 shha^{tbx392/tbx392} gli2a^ty119/+
Anatomical Terms:	adaxial cell fast muscle cell migratory slow muscle precursor cell muscle pioneer slow muscle cell somite
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 7(7), Hinits, Y., Osborn, D.P., Carvajal, J.J., Rigby, P.W., and Hughes, S.M., Mrf4 (myf6) is dynamically expressed in differentiated zebrafish skeletal muscle, 738-745, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns