ZFIN ID: ZDB-FIG-070821-19
Shimizu et al., 2005 - E-cadherin is required for gastrulation cell movements in zebrafish. Mechanisms of Development   122(6):747-763 Full text @ Mech. Dev.
Knockdown Reagent:
Observed In:
Stage: 75%-epiboly

Fig. 8 Cell adhesion between the deep cells and the enveloping layer is disrupted in cdh1 morphant embryos. Wild-type control (wt, A, C, E, G) and cdh1 morphant embryos (MO) embryos (B, D, F, H) were fixed at 8.5 hpf (80% epiboly for the wild-type) and subjected to electron microscopic analysis. Lateral regions. Adhesion between the enveloping layer (EVL) and the deep cells (DCs) was detected in the wild-type control embryos (indicated by arrows in A, C, E), but was severely affected in the cdh1 morphant embryos (arrows in B, D, F). In contrast, the adhesions between the EVL cells (arrowheads in A, C, D) or between the DCs (arrowheads in G, H) were not significantly affected in the cdh1 morphant embryos, compared with the wild-type control embryos.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO6-cdh1 standard conditions 75%-epiboly cell-cell adhesion involved in gastrulation decreased process quality, abnormal
75%-epiboly DEL detached from EVL, abnormal
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 122(6), Shimizu, T., Yabe, T., Muraoka, O., Yonemura, S., Aramaki, S., Hatta, K., Bae, Y.K., Nojima, H., and Hibi, M., E-cadherin is required for gastrulation cell movements in zebrafish, 747-763, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.