FIGURE

Fig. 6

ID

ZDB-FIG-070813-30

Publication

Etard et al., 2007 - The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis

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Fig. 6

Knockdown of hsp90a phenocopies the steif phenotype. (A, B) uninjected, (C, D) hsp90a, (E, F) hsp90a2, and (G, H) hsp90b morpholino injected embryos show normal birefringence at 72 hpf while hsp90a morpholino (C) injected embryos lack birefringence similar to steif mutants. (B, D, F, H) immunostaining of slow muscle myosin of control (B), hsp90a-morpholino (D), hsp90a2-morpholino (F), and hsp90b-morpholino injected embryos. Scale bar: 120 μm (A, C, E, G); 50 μm (B, D, F, H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-hsp90aa1.2 WT + MO2-hsp90aa1.1 WT + MO2-hsp90ab1
Knockdown Reagents:	MO1-hsp90aa1.2 MO2-hsp90aa1.1 MO2-hsp90ab1
Observed In:	heart contraction larval locomotory behavior slow muscle cell myosin filament trunk musculature whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 308(1), Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U., The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis, 133-143, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.