FIGURE

Fig. 5

ID

ZDB-FIG-070802-32

Publication

Kok et al., 2007 - The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

SBU2 mutants are not neurogenic. Whole mount RNA in situ hybridization of neural markers in wild-type and SBU2 mutants. (A–J) Dorsal views; (A–H) anterior to the top, (I, J) flat mounted, anterior to the right. Panels I′, J′ are lateral views of panels I and J, anterior to the top right. Morphology of the MHB domain expressing pax2a at early somitogenesis (B) indicates a delay in development of MHB in SBU2 mutants. Later, at the 10-somite stage (D) pax2a expression resembles much earlier stage wild-type expression domain (A). Likewise, although hindbrain marker krox20 expression is also delayed and relatively reduced (E and F) later in the development, expression pattern appears similar to wild-type embryos (G and H). RNA in situ hybridization using huC probe revealed that SBU2 embryos have reductions in early differentiating neurons (I, I′ and J, J′). Arrows denote MHB and arrowheads denote otic placode.

Expression Data

Genes:	egr2b elavl3 pax2a
Fish:	WT supt6h^sbu2/sbu2
Anatomical Terms:	hindbrain neural keel midbrain hindbrain boundary neural keel neuron otic placode presumptive rhombomere 3 presumptive rhombomere 5 rhombomere 3 rhombomere 5
Stage Range:	1-4 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 307(2), Kok, F.O., Oster, E., Mentzer, L., Hsieh, J.C., Henry, C.A., and Sirotkin, H.I., The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis, 214-226, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.