ZFIN ID: ZDB-FIG-070613-7
Shin et al., 2007 - Notch signaling regulates neural precursor allocation and binary neuronal fate decisions in zebrafish. Development (Cambridge, England)   134(10):1911-1920 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 5 Conditional activation of Notch signaling produces excess KA' interneurons at the expense of PMNs. (A-F) Lateral views of the spinal cord of zebrafish embryos at the 6- to 12-somite region, with anterior to the left and dorsal at the top. (A,B) Control Tg(UAS:Notch1aac-myc);Tg(olig2:egfp), (C,D) Tg(hsp70l:gal4vp16);Tg(UAS:Notch1aac-myc);Tg(olig2:egfp) and (E,F) Tg(hsp70l:XdnSu(H)myc);Tg(olig2:egfp) embryos were heat shocked at 11 hpf. (A,B) Control embryos showed normal numbers of isl2+ PMNs (black bracket), dorsal Rohon-Beard sensory neurons (white bracket) and GABAergic KA' cells (arrowheads). (C) isl2+ PMNs (black bracket) and Rohon-Beard neurons (white bracket) were almost absent from heat-shocked triple-transgenic embryos. (D) Heat-shocked triple-transgenic embryos had excess KA' interneurons (arrowheads). (E,F) Heat-shocked Tg(hsp70l:XdnSu(H)myc);Tg(olig2:egfp) embryos had excess PMNs (black bracket), excess Rohon-Beard neurons (white bracket) and a deficit of KA' interneurons (arrowheads). (G) Mean of the number of PMNs (gray bar) and KA' interneurons (black bar) per hemisegment (n=7) in A-F. Error bars represent s.e.m. Scale bar: 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl2a kca3Tg; kca4Tg; vu12Tg standard conditions 20-25 somites primary motor neuron ISH
kca3Tg; vu12Tg standard conditions 20-25 somites primary motor neuron ISH
vu12Tg; vu21Tg standard conditions 20-25 somites primary motor neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg/+; vu12Tg; vu22Tg heat shock 20-25 somites Rohon-Beard neuron decreased amount, abnormal
20-25 somites spinal cord motor neuron differentiation disrupted, abnormal
20-25 somites spinal cord primary motor neuron decreased amount, abnormal
Prim-5 spinal cord CNS interneuron decreased amount, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
vu12Tg; vu21Tg heat shock 20-25 somites Rohon-Beard neuron increased amount, abnormal
20-25 somites spinal cord motor neuron differentiation disrupted, abnormal
20-25 somites spinal cord primary motor neuron increased amount, abnormal
Prim-5 spinal cord CNS interneuron decreased amount, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development