Recovery of Notch signaling after transient inhibition. Dorsal views of flat-mounted zebrafish embryos, labeled by her4. A′-I′ are magnified views of boxed areas in A-I. (A,A′) Control embryo showing normal expression of her4 at 12 hpf. Bracket marks her4-expressing neuroectoderm cells. (B,B′) Continuous DAPT incubation caused significant reduction in her4 expression in posterior neuroectoderm (bracket). (C,C′) Embryos incubated with DAPT from 6 to 11 hpf re-expressed her4 (bracket) at 12 hpf. (D-F′) 11 hpf embryos. (D,D′) Control embryo. (E,E′) After heat shock at 9.5 hpf, Tg(hsp70l:XdnSu(H)myc) embryos did not express her4. (F,F′) After heat shock at 6 hpf, transgenic embryos did not express her4. (G-I′) 12 hpf embryos. (G,G′) Control embryo. (H,H′) After heat shock at 9.5 hpf, transgenic embryos did not express her4. (I,I′) After heat shock at 6 hpf, her4 expression was evident in posterior neuroectoderm in transgenic embryos. Scale bar: in A, 200 μm for A-I; in A′, 100 μm for A′-I′.
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