ZFIN ID: ZDB-FIG-070613-3
Shin et al., 2007 - Notch signaling regulates neural precursor allocation and binary neuronal fate decisions in zebrafish. Development (Cambridge, England)   134(10):1911-1920 Full text @ Development
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 2 Loss of Notch signaling causes formation of excess PMNs and a deficit of KA' interneurons. (A-D) Lateral views of the spinal cord at the 6- to 12-somite region of zebrafish embryos, with anterior to left and dorsal at the top. (A) A subset of PMNs (CaP and VaP) and Rohon-Beard neurons of Tg(olig2:egfp) embryos expressed isl2 in the ventral spinal cord (black bracket) and dorsal spinal cord (white bracket) at 20 hpf, respectively. (B) In Tg(olig2:egfp) embryos, KA' interneurons (arrowheads) were detected by anti-GABA antibody (red) within EGFP+ cells at 25 hpf. EGFP- GABA+ cells (arrows) in ventral spinal cord are KA'' interneurons (Park et al., 2004). (C) In mib-/-;Tg(olig2:egfp) embryos, isl2+ PMNs formed as clusters in each hemisegment in the ventral spinal cord (black bracket). (D) mib-/-;Tg(olig2:egfp) embryos did not produce EGFP+ GABA+ KA' interneurons, although EGFP- GABA+ KA'' interneurons were still present (arrows). (E) Mean of the number of PMNs (gray bar) and KA' interneurons (black bar) per hemisegment in Tg(olig2:egfp) and mib-/-;Tg(olig2:egfp) embryos (n=7). Error bars represent s.e.m. Scale bar: 50 ┬Ám.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mib1ta52b/ta52b; vu12Tg standard conditions 20-25 somites spinal cord motor neuron differentiation disrupted, abnormal
20-25 somites spinal cord primary motor neuron increased amount, abnormal
Prim-5 spinal cord CNS interneuron decreased amount, abnormal
Prim-5 ventral spinal cord interneuron differentiation disrupted, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development