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ZFIN ID: ZDB-FIG-070315-4
Wakahara et al., 2007 - fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish. Developmental Biology   303(2):527-535 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15

Fig. 6 Expression of tbx5 and fibin in pectoral fin buds during zebrafish embryonic development. The expression of tbx5 (a–e) and fibin (f–j) was examined in presumptive pectoral fin bud regions during different developmental stages (13∼30 hpf) by whole mount in situ hybridization. The expression of tbx5 and fibin in presumptive pectoral fin bud regions at 22 hpf was also examined at transverse sections (16 μm) (k, l). The expression of pax2.1 was examined in embryos at 14 hpf by whole mount in situ hybridization (m). The expression of pax2.1 and fibin was examined in embryos at 14 hpf by double whole mount in situ hybridization (n). The expression of tbx5 and fibin was also examined in presumptive pectoral fin bud regions at 24 hpf by double whole mount in situ hybridization (o). Arrowheads and arrows indicate the expression of tbx5 and fibin in presumptive pectoral fin bud regions, respectively. S1, S2 and S3 indicate the locations of somite 1, somite 2 and somite 3, respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fibinb WT standard conditions 5-9 somites lateral plate mesoderm ISH
10-13 somites lateral plate mesoderm ISH
10-13 somites somite 1 ISH
10-13 somites somite 2 ISH
10-13 somites somite 3 ISH
14-19 somites lateral plate mesoderm ISH
26+ somites lateral plate mesoderm ISH
Prim-15 Not Detected pectoral fin bud ISH
pax2a WT standard conditions 10-13 somites lateral plate mesoderm ISH
tbx5a WT standard conditions 5-9 somites lateral plate mesoderm ISH
14-19 somites posterior lateral plate mesoderm ISH
26+ somites posterior lateral plate mesoderm ISH
Prim-15 pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 303(2), Wakahara, T., Kusu, N., Yamauchi, H., Kimura, I., Konishi, M., Miyake, A., and Itoh, N., fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish, 527-535, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.