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ZFIN ID: ZDB-FIG-070315-2
Wakahara et al., 2007 - fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish. Developmental Biology   303(2):527-535 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to Prim-5

Fig. 3 Expression of fibin in zebrafish embryos. (A) The spatiotemporal expression pattern of fibin in zebrafish embryos (10∼24 hpf) was examined by whole mount in situ hybridization. fibin was expressed in the notochord at 10 hpf (a) and in the notochord and somites at 12 and 14 hpf (b, c). fibin was also expressed in the lateral plate mesoderm, tail buds and the forebrain at 14 hpf (c, d). At 18 and 24 hpf, fibin was predominantly expressed in tail buds and presumptive pectoral fin bud regions. In addition, fibin was also expressed in the alar plate (e, f). (B) The expression of fibin in fibin MO-injected zebrafish embryos. The expression of fibin in wild-type (a), antisense five-base mismatched fibin (Control) MO- (b) and antisense fibin MO- (c) injected embryos at 18 hpf was examined by whole mount in situ hybridization. 1, notochord; 2, somites; 3, lateral plate mesoderm or presumptive pectoral fin bud regions; 4, tail; 5, forebrain; 6, alar plate of the hindbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fibinb WT standard conditions Bud notochord ISH
5-9 somites forebrain neural rod ISH
5-9 somites lateral plate mesoderm ISH
5-9 somites notochord ISH
5-9 somites somite ISH
5-9 somites tail bud ISH
14-19 somites hindbrain ISH
14-19 somites lateral plate mesoderm ISH
14-19 somites posterior lateral plate mesoderm ISH
14-19 somites tail bud ISH
Prim-5 hindbrain ISH
Prim-5 lateral plate mesoderm ISH
Prim-5 posterior lateral plate mesoderm ISH
Prim-5 tail bud ISH
WT + MO1-fibinb standard conditions 14-19 somites posterior lateral plate mesoderm ISH
14-19 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 303(2), Wakahara, T., Kusu, N., Yamauchi, H., Kimura, I., Konishi, M., Miyake, A., and Itoh, N., fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish, 527-535, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.