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Fig. 4 Arhgef11 is not required for formation of Kupffer′s vesicle and cilia. (A,B) Posterior region of live, uninjected WT and MO-injected embryos. The inset is a magnified view of Kupffer′s vesicle. White scale bar, 100 μm; black scale bar, 25 μm. (C,D) Acetylated tubulin immunostaining of cilia within Kupffer′s vesicle. Scale bar, 10 μm. (E) Comparison of Kupffer′s vesicle cilia length for WT control, MO-injected (AUG, SPL) and vu7/vu7 embryos. The asterisk marks significantly different lengths (P≤0.001).
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