FIGURE

Fig. 2

ID

ZDB-FIG-070307-55

Publication

Stoick-Cooper et al., 2007 - Distinct Wnt signaling pathways have opposing roles in appendage regeneration

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Fig. 2

Wnt/β-catenin signaling is required for zebrafish tail fin regeneration. (A) Experimental scheme. Tail fins were amputated from wild-type, hsDkk1GFP or hsΔTcfGFP transgenic zebrafish and heat shocks were applied twice daily for the time periods indicated by the colored lines. (B) Continuous suppression of Wnt target gene expression in hsΔTcfGFP (n=15), or reduction of Wnt/β-catenin signaling in hsDkk1GFP transgenic fish (n=19), for 7 days starting shortly before amputation inhibits fin regeneration. Live fins were photographed at 1 dpa (left panels) and 7 dpa (right panels). (C) Overexpression of Dkk1 starting at 1 dpa inhibits fin regeneration (left panel; n=18); overexpression from 3 dpa results in partial inhibition of regeneration (right panel; 11 of 15 fins). Live fins were photographed at 7 dpa; corresponding wild-type controls regenerated normally (not shown).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w32Tg/+ w26Tg
Condition:	heat shock, physical alteration: anatomical structure
Observed In:	blastema fin regeneration regenerating fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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