ZFIN ID: ZDB-FIG-070307-55
Stoick-Cooper et al., 2007 - Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Development (Cambridge, England)   134(3):479-489 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 2 Wnt/β-catenin signaling is required for zebrafish tail fin regeneration. (A) Experimental scheme. Tail fins were amputated from wild-type, hsDkk1GFP or hsΔTcfGFP transgenic zebrafish and heat shocks were applied twice daily for the time periods indicated by the colored lines. (B) Continuous suppression of Wnt target gene expression in hsΔTcfGFP (n=15), or reduction of Wnt/β-catenin signaling in hsDkk1GFP transgenic fish (n=19), for 7 days starting shortly before amputation inhibits fin regeneration. Live fins were photographed at 1 dpa (left panels) and 7 dpa (right panels). (C) Overexpression of Dkk1 starting at 1 dpa inhibits fin regeneration (left panel; n=18); overexpression from 3 dpa results in partial inhibition of regeneration (right panel; 11 of 15 fins). Live fins were photographed at 7 dpa; corresponding wild-type controls regenerated normally (not shown).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w26Tg physical alteration: anatomical structure, heat shock Adult fin regeneration disrupted, abnormal
Adult regenerating fin decreased length, abnormal
w32Tg/+ physical alteration: anatomical structure, heat shock Adult blastema aplastic, abnormal
Adult fin regeneration disrupted, abnormal
Adult regenerating fin decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development