FIGURE

Fig. S2

ID

ZDB-FIG-061110-7

Publication

Berndt et al., 2006 - Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain

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Fig. S2

Rescue of the Sema3D splice morpholino (3DI4E5MO) by Sema3D^myc overexpression. (A-F) In situ hybridization for cyclin D1 in the hindbrain at 17 hpf in Sema3D^myc transgenic embryos (A-D) or wild-type embryos (E,F) that were uninjected (A,B) or injected with 3DI4E5MO (C-F) and were either not heat-shocked (A,C,E) or heat-shocked for 1 hour at 14 hpf (B,D,F). (G,H) Quantification of the number of BrdU (I) and PH3 (J) labeled nuclei in the neuroepithelium of rhombomeres 3-5. Numbers in parentheses indicate n. *P<0.005 versus uninjected NHS, ^#P<0.03 versus wild-type HS. oto, otocyst. (A-F) Dorsal views, anterior is leftwards. Scale bars: 40 μm for A-F.

Expression Data

Gene:	ccnd1
Fish:	WT + MO3-sema3d mi3Tg mi3Tg + MO3-sema3d
Condition:	heat shock
Knockdown Reagent:	MO3-sema3d
Anatomical Terms:	hindbrain proliferative region
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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