PUBLICATION

Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain

Authors
Berndt, J.D., and Halloran, M.C.
ID
ZDB-PUB-060921-15
Date
2006
Source
Development (Cambridge, England)   133(20): 3983-3992 (Journal)
Registered Authors
Berndt, Jason, Halloran, Mary
Keywords
Neural crest, Zebrafish, Semaphorin, TCF, Cell cycle, Cyclin
MeSH Terms
  • Animals
  • Rhombencephalon/chemistry
  • Rhombencephalon/cytology
  • Rhombencephalon/growth & development*
  • Wnt Proteins/metabolism
  • Cell Movement/genetics
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Cyclins/genetics
  • Pluripotent Stem Cells/chemistry
  • Pluripotent Stem Cells/cytology
  • Pluripotent Stem Cells/physiology*
  • Mutation
  • Semaphorins/antagonists & inhibitors
  • Semaphorins/genetics
  • Semaphorins/physiology*
  • Zebrafish/growth & development*
  • Cell Proliferation
  • Neural Crest/chemistry
  • Neural Crest/cytology
  • Neural Crest/growth & development*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Gene Expression
  • Nerve Growth Factors/antagonists & inhibitors
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/physiology*
  • Body Patterning
(all 29)
PubMed
16971468 Full text @ Development
Abstract
Neural crest cells (NCCs) are pluripotent migratory cells that are crucial to the development of the peripheral nervous system, pigment cells and craniofacial cartilage and bone. NCCs are specified within the dorsal ectoderm and undergo an epithelial to mesenchymal transition (EMT) in order to migrate to target destinations where they differentiate. Here we report a role for a member of the semaphorin family of cell guidance molecules in NCC development. Morpholino-mediated knockdown of Sema3d inhibits the proliferation of hindbrain neuroepithelial cells. In addition, Sema3d knockdown reduces markers of migratory NCCs and disrupts NCC-derived tissues. Similarly, expression of a dominant-repressor form of TCF (DeltaTCF) reduces hindbrain cell proliferation and leads to a disruption of migratory NCC markers. Moreover, expression of DeltaTCF downregulates sema3d RNA expression. Finally, Sema3d overexpression rescues reduced proliferation caused by DeltaTCF expression, suggesting that Sema3d lies downstream of Wnt/TCF signaling in the molecular pathway thought to control cell cycle in NCC precursors.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mi3TgTransgenic Insertion
    w26TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      sema3dMO2-sema3dMRPHLNO
      sema3dMO3-sema3dMRPHLNO
      sema3dMO4-sema3dMRPHLNO
      1 - 3 of 3
      Show
      Fish
      Fish
      AB
      AB + MO2-sema3d
      AB + MO3-sema3d
      AB + MO4-sema3d
      mi3Tg
      mi3Tg + MO3-sema3d
      w26Tg (AB)
      WT
      WT + MO3-sema3d
      1 - 9 of 9
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Berndt, J.D., and Halloran, M.C. (2006) Semaphorin 3d promotes cell proliferation and neural crest cell development downstream of TCF in the zebrafish hindbrain. Development (Cambridge, England). 133(20):3983-3992.