ZFIN ID: ZDB-FIG-060912-11
Kazakova et al., 2006 - A screen for mutations in zebrafish that affect myelin gene expression in Schwann cells and oligodendrocytes. Developmental Biology   297(1):1-13 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 5  PLL phenotype of nls and nof mutants. (A) 5 dpf nls mutant fish. Note mbp expression in the anterior lateral line (arrows) but absent from the PLL. (B) PLL nerve (arrow) of nls mutant 5 dpf stained with anti-acetylated tubulin. In the wild type, axons branch off ventrally at intervals to innervate individual neuromeres (arrowhead) but no such branching is seen in the mutant. (C, D) 5 dpf nof mutant fish. The nof phenotype shows variable expressivity and in some mutants mpb is expressed on one side only (arrow) as shown in panel D. Note normal mbp expression in the PLL on the left side (arrow). (E, F) Plp1b expression in nls mutant (E) and wild type (F) fish. Plp1b is detectable on both sides in the wild type and on one side of the nls mutant (arrow). (G. H) Electron micrographs of the PLL in nls mutant fish. Note loosely wrapped ensheathment by myelin (arrows) of the large diameter axons. Scale bars in panels B, E and F, 10 μm; in inset in panel B, 5 μm; in panel G 1 μm; in panel H, 200 nm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mbpa aldh1a2i26/i26 standard conditions Day 5 anterior lateral line ISH
Day 5 Not Detected posterior lateral line ISH
aldh1a2u11/u11 standard conditions Day 5 posterior lateral line anatomical side ISH
plp1b WT standard conditions Day 5 posterior lateral line ISH
aldh1a2i26/i26 standard conditions Day 5 posterior lateral line ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aldh1a2i26/i26 standard conditions Day 5 axonogenesis involved in innervation decreased process quality, abnormal
Day 5 posterior lateral line nerve dendrite unbranched, abnormal
Day 5 posterior lateral line nerve development process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 297(1), Kazakova, N., Li, H., Mora, A., Jessen, K.R., Mirsky, R., Richardson, W.D., and Smith, H.K., A screen for mutations in zebrafish that affect myelin gene expression in Schwann cells and oligodendrocytes, 1-13, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.