FIGURE

Fig. 4

ID

ZDB-FIG-060828-10

Publication

Raeker et al., 2006 - Obscurin is required for the lateral alignment of striated myofibrils in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Suppression of obscurin expression in MO2-treated embryos. A: Embryos injected with MO2 (MO2; 3 and 6 ng) or a control morpholino (CMO2; 6 ng) were examined at 72 hpf. Note that the morphant embryos are slightly shorter with diminished regularity and definition of the transverse myoseptae (>) and moderate pericardial edema (<). Most 72-hpf MO2-treated embryos demonstrated a looped but hypoplastic heart with a small, underdeveloped ventricular (v) chamber compared to control. B: Phenotypic effects of injection with MO1, MO2, or the corresponding control morpholinos, CMO1 and CMO2. Embryos were phenotyped at 72 hpf and categorized as having cardiac and/or somite defects. All embryos injected with control morpholinos at these dosages were morphologically normal. C: Suppression of obscurin expression in MO2-injected embryos (6-ng dose). MO2-injected 72-hpf embryos demonstrate reduced immunostaining for obscurin (Rho Ab) compared to controls using identical reaction conditions.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO2-obscnb
Knockdown Reagent:	MO2-obscnb
Observed In:	cardiac ventricle heart pericardium striated muscle cell development vertical myoseptum whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.