ZFIN ID: ZDB-FIG-060808-227
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 14-19 somites

Fig. 6 Sfrp compensates for inhibition of Lhx5 function.(A) Sfrp1a and Sfrp5 rescue eye development in lhx5-en mRNA-injected embryos. The percentages of embryos malformed, as a result of dorsalization or injection (yellow), eyeless (blue) or two eyed (green) were measured. (B-E) Ectopic Wnt signaling results in ectopic axin2 expression. (B,C) Animal pole views; (D,E) lateral views, rostral to the left, dorsal to the top. Genotypes were determined by RFLP. (F-I) Ectopic axin2 expression in lhx5 morpholino-injected embryos. Flat mounts in glycerol. Brain boundaries are marked by broken lines. Green arrows indicate the borders of axin2 labeling. (F,G) Dorsal views, rostral to the top; (H,I) ateral views, rostral to the left, dorsal to the top. Eyes were removed to expose the brain. (J-M) sfrp1a or sfrp5 gain of function reduces ectopic axin2 expression in lhx5-en-injected embryos. Animal pole views, dorsal to the left. Scale bar in M: 250 μm for B,C; 125 μm for D,E; 100 μm for F-I; 250 μm for J-M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Shield margin ISH
1-4 somites forebrain neural keel ISH
14-19 somites brain ISH
WT + MO1-lhx5 standard conditions 14-19 somites brain ISH
14-19 somites forebrain ISH
axin1tm213/tm213 standard conditions 1-4 somites forebrain neural keel ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development