ZFIN ID: ZDB-FIG-060726-3
Brand et al., 1996 - Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development (Cambridge, England)   123:179-190 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 1 Lateral views of living noi and ace mutant embryos. (A) Wild-type embryo at 36 hours of development. (B) Sibling of the embryo in A that is homozygous mutant for a strong allele of noi (noith44). Absence of the tectum, cerebellum and MHB is indicated by an asterisk. (C) Embryo homozygous mutant for a weak allele of noi (noity31a). A partially formed tectum, but no cerebellum or MHB, is observed. (D) Embryo homozygous mutant for acerebellar (aceti282a). An enlarged tectum is present, but no cerebellum or MHB are seen (see also Fig. 7). (E) Pronephric duct (pn) in a wild type and (F) homozygous mutant embryo for noith44. In the mutant embryos, no pronephric duct is found; the arrow points to the few cells in the mutant that may represent partially developed pronephric duct cells; consistent with the presence of a normal duct during earlier stages of development. (G) Blood streaming through the common cardinal vein over the yolk in a 36 h wild-type embryo (bracketed by arrows). (H) Blood cells accumulate in a dent on the ventral yolk sac in a homozygous mutant embryo for noith44. No such accumulation is seen in wild-type embryos. Embryos in A and B and E-F were placed in PTU at about 26 h to suppress formation of melanin. cb, cerebellum; ey, eye; tec, tectum; ov, otic vesicle; pn, pronephric duct. Scale bar, 150 µm (A-D), 60 µm (E-H).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf8ati282a/ti282a standard conditions Prim-25 blood circulation disrupted, abnormal
Prim-25 cerebellum absent, abnormal
Prim-25 midbrain hindbrain boundary absent, abnormal
Prim-25 optic tectum increased size, abnormal
pax2ath44/th44 control Prim-25 blood circulation disrupted, abnormal
Prim-25 cerebellum absent, abnormal
Prim-25 midbrain hindbrain boundary absent, abnormal
Prim-25 optic tectum absent, abnormal
Prim-25 otic vesicle morphology, abnormal
Prim-25 pronephric duct absent, abnormal
pax2aty31a/ty31a standard conditions Prim-25 cerebellum absent, abnormal
Prim-25 midbrain hindbrain boundary absent, abnormal
Prim-25 optic tectum decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development