ZFIN ID: ZDB-FIG-060726-5
Brand et al., 1996 - Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain. Development (Cambridge, England)   123:179-190
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 3 Overall organisation of the CNS in noi mutant embryos. Shown are dorsal views of wild type and mutant embryos stained with antibodies to reveal axonal organisation and formation of neuronal nuclei. (A,B) Anti-acetylated-tubulin staining in 30 h wild-type and mutant noity22b embryos. Arrowheads point to the position of the tract of the posterior commissure, and to the first hindbrain commissure. Notice the reduced distance. The arrow in A points to the ventral tegmental commissure that is absent in the mutant noi embryo in B. (C,D) mAb 3A10 staining in 30 h wild-type and mutant noith44 embryos. The Mauthner neuron (mn) and the other T reticular interneurons stained by this antibody develop normally, indicating that hindbrain development is largely normal in noi embryos. (E,F) Isl staining of 32 h wild-type and mutant noith44 embryos. Arrowheads point to bilateral pairs of clustered neuronal nuclei stained by this antibody in the midbrain and hindbrain (see text). Whereas two clusters are seen in the midbrain of the wild-type embryo in E (probably corresponding to the nucleus of the medial longitudinal fascicle), only one such pair of reduced size is seen in the mutant. Notice the reduced distance to the rhombomere 2 cluster. A group of 2-3 nuclei in rhombomere 1 is also unaffected in the mutant, but not in focus. trg, trigeminal ganglion. Scale bar, 100 µm (A-D), 75 µm (E, F).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-3A10 IHC WT standard conditions Prim-15 Mauthner neuron
IHC Prim-15 T interneuron
IHC pax2ath44/th44 standard conditions Prim-15 Mauthner neuron
IHC Prim-15 T interneuron
Ab1-isl IHC WT standard conditions Prim-15 CNS neuron (sensu Vertebrata) nucleus
IHC pax2ath44/th44 standard conditions Prim-15 CNS neuron (sensu Vertebrata) nucleus
Ab1-tuba IHC WT standard conditions Prim-15 ansulate commissure
IHC Prim-15 medial longitudinal fasciculus
IHC pax2aty22b/ty22b standard conditions Prim-15 Not Detected ansulate commissure
IHC Prim-15 medial longitudinal fasciculus
Phenotype Details
Fish Conditions Stage Phenotype
pax2ath44/th44 standard conditions Prim-15 cranial nerve IV aplastic, abnormal
Prim-15 oculomotor nucleus has fewer parts of type neuron, abnormal
Prim-15 trochlear motor nucleus has fewer parts of type neuron, abnormal
pax2aty22b/ty22b standard conditions Prim-15 ansulate commissure decreased size, abnormal
Prim-15 forebrain midbrain boundary decreased distance hindbrain commissure, abnormal
Acknowledgments:
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