ZFIN ID: ZDB-FIG-060712-1
Niikura et al., 2006 - Zebrafish Numb homologue: Phylogenetic evolution and involvement in regulation of left-right asymmetry. Mechanisms of Development   123(5):407-414 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1k-cell to Adult

Fig. 2 Expression of znumb, notch1a, deltaD in zebrafish development. (A) Semi-quantitative RT-PCR analysis of znumb mRNA expression. Total RNA was isolated from various tissues of adult zebrafish, and znumb transcripts were amplified by using specific primers. μ-actin was used as a control. (B) Whole-mount in situ hybridization patterns of znumb expression during zebrafish embryonic development. Zebrafish embryos at various stages as indicated were hybridized with the antisense probe. Upper (3 hpf) panels are lateral views with the animal pole at the top. For the second (12 hpf) and third (15 hpf) rows, left panels are lateral view of embryos with anterior to the left, middle panels are frontal view with dorsal at the top, and right panels are a view of embryos from the posterior with dorsal at the top. A panel of 20 hpf in the fourth row is a lateral view with anterior to the left. Enlarged image is shown to the right side. (C,D) Whole-mount in situ hybridization patterns of notch1a (C) and deltaD (D). Left panels are lateral view of embryos with anterior to the left, middle panels are frontal view with dorsal at the top, and right panels are views from caudal to anterior with dorsal at the top. nk, neural keel; t, telencephalon; d, diencephalon; m, midbrain; h, hindbrain; kv, Kupffer′s vesicle; s, somites; tb, tail bud; nr, neural retina; sc, spinal cord. Sense probe hybridization was done for all samples, and no significant signals were observed (data not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dld WT standard conditions 5-9 somites somite ISH
5-9 somites tail bud ISH
10-13 somites somite ISH
10-13 somites tail bud ISH
notch1a WT standard conditions 5-9 somites central nervous system ISH
5-9 somites Kupffer's vesicle ISH
10-13 somites central nervous system ISH
10-13 somites tail bud ISH
numbl WT standard conditions 1k-cell whole organism ISH
30%-epiboly whole organism ISH
90%-epiboly whole organism ISH
5-9 somites central nervous system ISH
10-13 somites brain ISH
10-13 somites immature eye ISH
20-25 somites brain ISH
20-25 somites immature eye ISH
Adult brain RTPCR
Adult eye RTPCR
Adult Not Detected heart RTPCR
Adult integument RTPCR
Adult kidney RTPCR
Adult Not Detected liver RTPCR
Adult musculature system RTPCR
Adult ovary RTPCR
Adult spleen RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 123(5), Niikura, Y., Tabata, Y., Tajima, A., Inoue, I., Arai, K.I., and Watanabe, S., Zebrafish Numb homologue: Phylogenetic evolution and involvement in regulation of left-right asymmetry, 407-414, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.