Fig. 4

Pard3 immunolocalization in has and nok mutant retinas. Immunohistochemistry was performed on 33 hpf (A–C) and 4 dpf (D–F) has^m567 mutant retinas, 4 dpf wild-type retinas (G–I), and 33 hpf nok^m520 mutant retinas (J–L) with anti-Pard3 antiserum (left column) and Alexa-488-conjugated phalloidin (middle column). The merged images are shown in the right column. Pard3 (A, arrows) localized to the apical region in the has^m567 retinal neuroepithelium at 33 hpf and partially overlapped with adherens junction-associated actin bundles. At 4 dpf, Pard3 and actin were both localized in the inner plexiform layer in the has^m567 retina (D, arrows), while actin staining in the outer plexiform layer was interrupted (E, arrowheads). In the 4 dpf wild-type retina, Pard3 and actin localized to the outer limiting membrane (arrowhead 1), the outer plexiform layer (arrowhead 2), and two sublayers in the inner plexiform layer (arrowheads 3 and 4). In the nok^m520 mutant retinal neuroepithelium, Pard3 localized to the ectopic adherens junction-associated actin bundles (K, arrows). Scale bars indicate 20 μm.