Fig. 2

Immunoblot of Pard3 proteins. Immunoblots of protein extracts from 27 hpf embryos that were incubated with either the anti-Pard3 polyclonal antiserum (A) or anti-γ-tubulin monoclonal antibody (B), which was used as a control to confirm that equivalent amounts of protein were loaded on each lane. The lanes correspond to wild-type embryos (lane 1), embryos injected with anti-pard3 Morph-1 (lane 2), embryos co-injected with Morph-1 and mRNA encoding the 180-kDa Pard3 isoform (lane 3), embryos co-injected with Morph-1 and mRNA encoding the 150-kDa Pard3 isoform (lane 4), embryos co-injected with Morph-1 and mRNA encoding the 150-kDa Pard3 isoform fused to a six-repeat myc-epitope (which increases the protein by approximately 9 kDa, lane 5), and embryos co-injected with Morph-1 and a mRNA encoding GFP (lane 6). All mRNAs contain the β-globin 5′ and 3′ UTRs flanking the open reading frame and were transcribed in vitro. The 180- and 150-kDa Pard3 proteins are marked with arrows. The location of the BioRad molecular weight markers (in kDa) is shown to the left.