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ZFIN ID: ZDB-FIG-060627-4
Sieger et al., 2006 - her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo. Developmental Biology   293(1):242-251 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Bud

Fig. 4 Disruption of early oscillations in her1/her13.2 morphants. Oscillations in gene expression of her1, her7, and deltaC are disrupted in her1/her13.2 morphants at bud stage. (A, E, and I) Wild-type expression of her1, her7, and deltaC, respectively. Dynamic gene expression can be found for her1 (B), her7 (F), and deltaC (J) in her1 morphants (0.6 mM; 2 experiments, n = 40 for each probe, all embryos show wild-type dynamics) since in a batch of embryos the whole variety of the pattern can be found (two patterns are shown each for wt, her1 morphant and her13.2 morphant situation, respectively). The same dynamic is seen in her13.2 morphants for her1 (C), her7 (G), and deltaC (K) (0.6 mM; 2 experiments, n = 45 for each probe, all embryos show wild-type dynamics). In her1/her13.2 morphants, a full disruption of cyclic her1 (D), her7 (H), and deltaC (L) expression is detected, and the whole batch of embryos shows only one pattern (0.6 mM her1Mo + 0.6 mM her13.2Mo; 2 experiments, n = 52 for each probe, 96.15% affected for her1, 94.23% affected for her7, and 92.3% affected for deltaC). (A–L) Bud stage embryos, whole mounts, dorsal view, posterior downwards.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
WT + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
her1 WT standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
WT + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
her7 WT standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 standard conditions Bud presumptive segmental plate ISH
WT + MO2-her1 + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
WT + MO3-hes6 standard conditions Bud presumptive segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 293(1), Sieger, D., Ackermann, B., Winkler, C., Tautz, D., and Gajewski, M., her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo, 242-251, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.