ZFIN ID: ZDB-FIG-060119-12
Gamse et al., 2005 - Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target. Development (Cambridge, England)   132(21):4869-4881 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. S3 Southpaw (Spaw) regulates the direction of epithalamic asymmetry. Spaw MO-depleted larvae show either a  WT (sinistral; A,C,E,G,H) or  reversed (dextral; B,D,F,I,J) pattern of epithalamic laterality.  (A,B) Dorsal view of fluorescently labeled pineal complex in foxD3:GFP transgenic larvae at 2 days. Position of the parapineal is indicated (white arrowhead). (C,D) Dense neuropil in the habenular nuclei is revealed by confocal imaging of anti-acetylated tubulin immunofluorescence in a 4-day larva. (E,F) Asymmetric expression pattern of the lov gene at 4 days. (G-J) Whole-mount in situ hybridization to detect lov habenular expression (open arrowhead) and insulin transcripts in the pancreas (black arrowhead) reveals the four major classes of larvae obtained following spaw MO injection. The direction of habenular asymmetry and L-R positioning of the pancreas are randomized, but in an independent manner. All images are dorsal views, anterior to the top. Scale bar: 8 mm (A,B); 20 mm (C,D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kctd12.1 WT + MO2-spaw standard conditions Day 4 habenula ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-spaw standard conditions Day 4 determination of left/right symmetry disrupted, abnormal
Day 4 determination of pancreatic left/right asymmetry disrupted, abnormal
Day 4 habenula symmetry, abnormal
Day 4 pancreas symmetry, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development