ZFIN ID: ZDB-FIG-051215-3
Etard et al., 2005 - Mutation in the delta-subunit of the nAChR suppresses the muscle defects caused by lack of Dystrophin. Developmental dynamics : an official publication of the American Association of Anatomists   234(4):1016-1025 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4 Expression of acetylcholine receptor (AChR) subunits in wild-type and sopfixe fish. A-E: Expression of the δ-subunit in wild-type (A-D) and sopfixe mutant (E) embryos. The δ-subunit is expressed exclusively in muscles. No differences in expression levels between wild-type and sopfixe mutants were detected (compare D with E; A, 24 hpf; B-E, 50 hpf). F,G,I,J: Comparison of the expression of the α1-subunit (F,I) and the γ-subunit (G,J) at 72 hpf. In contrast to all other subunits analyzed, the α1-subunit does not continue to be expressed in eye muscles after 50 hpf (compare F, I, arrow, with G,J). H,K: Schematic drawing of the muscles in the head in lateral (H) and ventral (K) views. ah, adductor hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; hh, hyohyoideus; hp, hypaxial muscles; ih, interhyoideus; ima, intramandibular anterior; imp, intramandibular posterior; io, inferior oblique; ir, inferior rectus; lap, levator arcus palatini; lr, lateral rectus; mr, medial rectus; pf, pectoral fin; sh, sternohyoideus; so, superior oblique; tv, transversus ventralis. A,C,F,G,H: Lateral view; B: dorsal view; D,E,I,J,K: ventral view. Scale bars = 80 μm in A, 60 μm in B-E, 50 μm in F-J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
chrna1 WT standard conditions Protruding-mouth Not Detected extraocular musculature ISH
Protruding-mouth Not Detected heart ISH
Protruding-mouth musculature system ISH
chrndsb13/sb13 standard conditions Protruding-mouth Not Detected extraocular musculature ISH
Protruding-mouth Not Detected heart ISH
Protruding-mouth musculature system ISH
chrnd WT standard conditions Prim-5 Not Detected primitive heart tube ISH
Prim-5 somite ISH
Long-pec Not Detected heart ISH
Long-pec musculature system ISH
chrndsb13/sb13 standard conditions Long-pec Not Detected heart ISH
Long-pec musculature system ISH
chrng WT standard conditions Protruding-mouth Not Detected heart ISH
Protruding-mouth musculature system ISH
chrndsb13/sb13 standard conditions Protruding-mouth Not Detected heart ISH
Protruding-mouth musculature system ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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