FIGURE

Fig. 5

ID

ZDB-FIG-051031-5

Publication

Challa et al., 2005 - Robo3 isoforms have distinct roles during zebrafish development

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Fig. 5

RNA expression patterns of robo3var1 and robo3var2 during the first day of development. Whole-mount RNA in situ hybridization for robo3var1 (A, C, E, G, I) and robo3var2 (B, D, F, H, J). robo3var1 RNA is expressed in trunk somites (A; ∼17 hpf), and at higher magnification (E, arrows) can be seen in the posterior aspect of the somites between 14 and 24 hpf. In contrast, robo3var2 is not expressed in the somites (B; ∼17 hpf) but is seen in the hindbrain (D, arrows, compare to C (dorsal views); ∼17 hpf) and spinal cord (F, arrows; ∼17 hpf) between 14 and 24 hpf. At 24 hpf, robo3var1 is expressed diffusely in the ventral spinal cord (G, arrows), whereas robo3var2 expression is more distinct (H, arrows). Cross-sections of the trunk at 26 hpf reveals that robo3var1 is expressed in ventral and intermediate spinal cord cells (I, arrows), whereas robo3var2 is expressed in dorsal and intermediate spinal cord cells (J, arrows). Anterior is to the left in A–H and dorsal is to the top in I and J.

Expression Data

Gene:	robo3
Fish:	WT
Anatomical Terms:	hindbrain neural rod neural tube somite spinal cord
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 122(10), Challa, A.K., McWhorter, M.L., Wang, C., Seeger, M.A., and Beattie, C.E., Robo3 isoforms have distinct roles during zebrafish development, 1073-1086, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.