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ZFIN ID: ZDB-FIG-050817-2
Sonawane et al., 2005 - Zebrafish penner/lethal giant larvae 2 functions in hemidesmosome formation, maintenance of cellular morphology and growth regulation in the developing basal epidermis. Development (Cambridge, England)   132(14):3255-3265 Full text @ Development
Anatomical Term:
Stage: Day 5

Fig. 2 Analysis of the pen mutant phenotype. (A,B) DIC images of 5-day-old wild type (A) and pen mutant larvae (B). The pen mutant larvae exhibit small ventral epidermal growths (arrowheads in B). (C,D) Giemsa stained thin sections of 5-day-old wild-type and mutant larvae. Detachment of epidermis is observed in mutant larvae (D). (E-H) Electron microscopic analysis of the epidermis. Hemidesmosomes are absent and collagen lamella is non-coherent (star) but the basal lamina in between appears normal in the epidermis of pen mutant larvae (E; also compare with Fig. 1E). Desmosomes (arrows in F), however, are present in the mutant epidermis. Tight junctions in the periderm of wild-type and pen mutant (arrowheads in G and H, respectively) show no ultra-structural differences. (I,J) Immunostaining with anti-ß-catenin antibody to mark adherens junctions. In wild type (I) as well as in mutant (J), ß catenin (arrowheads) is localised at the apical and lateral borders of basal epidermal cells. Staining intensity is lower in the pen mutant. Scale bar: 0.3 mm in A,B; 160 µm in C,D; 543 nm in E; 405 nm in F; 297 nm in G,H; 30 µm in I,J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnnb1 WT standard conditions Day 5 epidermis IHC
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development