ZFIN ID: ZDB-FIG-050725-1
Cha et al., 2005 - Cyclooxygenase-1 signaling is required for vascular tube formation during development. Developmental Biology   282(1):274-283 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Prim-5

Fig. 1 cox1 transcripts localize to posterior mesoderm organs. Whole mount in situ hybridization of cox1 (A–H) reveals that its transcripts are ubiquitously present at shield stage (A) and localize posterior half of the embryos at tailbud stage (B–C) from side view (B) and from posterior view (C). cox1 co-localizes with pax2 (H) and fli1 (G) at 7 somite stage, indicating its expression in posterior intermediate mesoderm and posterior lateral mesoderm. During late somitogenesis stage, cox1 transcripts strongly stain posterior mesoderm (D). At 24 hpf, cox1 can be seen in various mesodermal structures (E). Expression of cox1 is not altered in casanova mutant, deficient in endoderm derivatives (F). Arrow indicates the distal end of pronephric duct, letter v indicates the vasculature and arrowhead points to the distal hypochord. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fli1 WT standard conditions 5-9 somites intermediate mesoderm ISH
5-9 somites lateral plate mesoderm ISH
pax2a WT standard conditions 5-9 somites intermediate mesoderm ISH
5-9 somites lateral plate mesoderm ISH
ptgs1 WT standard conditions Shield whole organism ISH
Bud whole organism ISH
5-9 somites intermediate mesoderm ISH
5-9 somites lateral plate mesoderm ISH
14-19 somites mesoderm ISH
Prim-5 blood vasculature ISH
Prim-5 hypochord ISH
Prim-5 notochord ISH
Prim-5 pronephric duct ISH
sox32unspecified standard conditions Prim-5 blood vasculature ISH
Prim-5 hypochord ISH
Prim-5 notochord ISH
Prim-5 pronephric duct ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 282(1), Cha, Y.I., Kim, S.H., Solnica-Krezel, L., and Dubois, R.N., Cyclooxygenase-1 signaling is required for vascular tube formation during development, 274-283, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.