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ZIRC
ZFIN ID: ZDB-FIG-050425-5
Essner et al., 2005 - Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut. Development (Cambridge, England)   132(6):1247-1260 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to 5-9 somites

Fig. 5 ntl and the Nodal signaling pathway regulate lrdr1 expression, and, with other genes, control morphogenesis of Kupffer′s vesicle. Wild-type (A,F) and mutant ntlb195(B,G), sptb104(C,H), casta56 (D,I) and oepm134 (E,J) embryos were examined for lrdr1 mRNA expression, cilia formation and KV organization. (A-E) lrdr1 expression at 80-90% epiboly. Lateral views, dorsal towards the right, anterior upwards; arrows indicate DFCs. (F-J) Immunofluorescence analysis of cilia and KV morphogenesis using anti-acetylated Tubulin antibodies (cilia, green) and anti-Ntl antibodies (KV, red) at 6-10 SS. Dorsal views with anterior towards the left. Arrows indicate cilia.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dnah9 WT standard conditions 75%-epiboly forerunner cell group ISH
sox32ta56/ta56 standard conditions 75%-epiboly Not Detected forerunner cell group ISH
tbx16b104/b104(AB) standard conditions 75%-epiboly forerunner cell group ISH
tbxtab195/b195 standard conditions 75%-epiboly Not Detected forerunner cell group ISH
tdgf1m134/m134(AB) standard conditions 75%-epiboly Not Detected forerunner cell group ISH
tbxta WT standard conditions 5-9 somites Kupffer's vesicle IHC
5-9 somites notochord IHC
sox32ta56/ta56 standard conditions 5-9 somites notochord IHC
tbx16b104/b104(AB) standard conditions 5-9 somites notochord IHC
tdgf1m134/m134(AB) standard conditions 5-9 somites notochord IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development