ZFIN ID: ZDB-FIG-050323-29
Waxman et al., 2004 - Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Development (Cambridge, England)   131(23):5909-5921 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 dpr2 morphants phenocopy strabismus/trilobite (stbm) morphants, and functionally interact with stbm and wnt11/silberblick. (A) dpr2 morphants (middle-injected with 7.5 ng dpr2 MO) phenocopy stbm morphants (right-injected with 3 ng). Arrow indicates flattened, non-chevron-shaped somite. Arrowhead indicates narrowed eyes. (B) Scoring of embryos that were co-injected with suboptimal doses of stbm and dpr2 MOs. 0, wild type; 1, moderate CE defects; 2, severe CE defects. (C) Percentage of embryos injected with MOs exhibiting phenotype. (D) Scoring of cyclopia index of embryos co-injected with dpr2 MO and wnt11 MO (a-f). dpr2 MO (normal dose, g,h). 0, wild type; 1, narrowing of the eyes; 2, fusion of the lens. (E) Percentage of embryos injected displaying respective phenotypes. Suboptimal (hypomorphic) dose of dpr2 MO is 3 ng, stbm MO is 0.4 ng and wnt11 MO is 1.5 ng. All injections were balanced with control MO. The numbers in brackets indicate the numbers of embryos used.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dact2 standard conditions Prim-5 convergent extension involved in axis elongation disrupted, abnormal
Prim-5 eye decreased distance eye, abnormal
Prim-5 eye decreased width, abnormal
Prim-5 eye mislocalised anteriorly, abnormal
Prim-5 somite flat, abnormal
Prim-5 whole organism decreased length, abnormal
WT + MO1-dact2 + MO1-vangl2 standard conditions Prim-5 convergent extension involved in axis elongation disrupted, abnormal
Prim-5 eye decreased distance eye, abnormal
Prim-5 eye decreased width, abnormal
Prim-5 somite flat, abnormal
Prim-5 whole organism decreased length, abnormal
WT + MO1-dact2 + MO1-wnt11f2 standard conditions Prim-5 convergent extension involved in axis elongation disrupted, abnormal
Prim-5 eye fused with eye, abnormal
WT + MO1-vangl2 standard conditions Prim-5 convergent extension involved in axis elongation disrupted, abnormal
Prim-5 eye decreased distance eye, abnormal
Prim-5 eye decreased width, abnormal
Prim-5 somite flat, abnormal
Prim-5 whole organism decreased length, abnormal
WT + MO1-wnt11 standard conditions Prim-5 eye fused with eye, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development