FIGURE

Fig. 3

ID

ZDB-FIG-050221-10

Publication

Kajimura et al., 2005 - Insulin-like growth factor-binding protein-1 (IGFBP-1) mediates hypoxia-induced embryonic growth and developmental retardation

Other Figures

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Fig. 3

Knockdown of functional IGFBP-1 with splice-site-targeting MOs. (A) Structure of the zebrafish IGFBP-1 gene. Exons are shown as boxes, and introns are shown as lines. The splice donor site targeted by the IGFBP-1 MO is underlined. The primer set used for RT-PCR is shown as arrows. F, forward primer; R, reverse primer. (Scale bar, 1 kb.) (B) Dose-dependent effect after 0.5, 1, 2, 4, 8, or 16 ng MO1 was injected into embryos. The specific primer set amplified a 387-bp spliced form of IGFBP-1, whereas MO knockdown caused an aberrant splice form (1,008 bp). WT, wild-type; Cont, control MO; P.C., positive control of PCR; N.C., negative control of PCR. (C) Time-course effect. MO1 at dose of 2 ng was injected into embryos, and the knockdown effect was analyzed by RT-PCR at 30, 48, 72, and 96 h after the injection. (D) Sequence analysis of the IGFBP-1 cDNAs isolated from the wild-type and MO1-injected embryos. The sequence of the aberrantly spliced form is italicized. Note that the aberrant splicing form results in a coding-frame shift and a premature stop codon.

Expression Data

Gene:	igfbp1a
Fish:	WT WT + MO1-igfbp1a
Knockdown Reagent:	MO1-igfbp1a
Anatomical Term:	unspecified
Stage Range:	Prim-15 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA