CRISPR

CRISPR2-eif4eb

ID

ZDB-CRISPR-260626-6

Name

CRISPR2-eif4eb

Previous Names

None

Target

eif4eb (1)

Sequence

5' - CTCCAAATTTGACACCGTAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
os82	eif4eb
os83	eif4eb
os84	eif4eb
os85	eif4eb

Expression

Gene expression in Wild Types + CRISPR2-eif4eb

No data available

Phenotype

Phenotype resulting from CRISPR2-eif4eb

No data available

Phenotype of all Fish created by or utilizing CRISPR2-eif4eb

Phenotype	Fish	Conditions	Figures
heart regenerating tissue Ab5-mef2 labeling increased amount, abnormal	eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell 2-D shape, abnormal	eif4eb^os83/os83	standard conditions	Fig. 7. from Dockery et al., 2026
regenerating fin Ab5-mef2 labeling decreased amount, abnormal	eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell cell division increased occurrence, abnormal	eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
heart regenerating tissue Ab5-mef2 labeling increased amount, abnormal	eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell 2-D shape, abnormal	eif4eb^os84/os84	standard conditions	Fig. 7. from Dockery et al., 2026
regenerating fin Ab5-mef2 labeling decreased amount, abnormal	eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell cell division increased occurrence, abnormal	eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
heart Ab5-mef2 labeling decreased distribution, abnormal	eif4e1c^pd370/pd370; eif4eb^os83/os83	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism decreased mass, exacerbated	eif4e1c^pd370/pd370; eif4eb^os83/os83	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism shortened, exacerbated	eif4e1c^pd370/pd370; eif4eb^os83/os83	standard conditions	Fig. 4. from Dockery et al., 2026
cardiac muscle cell decreased amount, exacerbated	eif4e1c^pd370/pd370; eif4eb^os83/os83	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism viability, abnormal	eif4e1c^pd370/pd370; eif4eb^os83/os83	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism decreased mass, exacerbated	eif4e1c^pd370/pd370; eif4eb^os84/os84	standard conditions	Fig. 4. from Dockery et al., 2026
heart Ab5-mef2 labeling decreased distribution, abnormal	eif4e1c^pd370/pd370; eif4eb^os84/os84	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism shortened, exacerbated	eif4e1c^pd370/pd370; eif4eb^os84/os84	standard conditions	Fig. 4. from Dockery et al., 2026
cardiac muscle cell decreased amount, exacerbated	eif4e1c^pd370/pd370; eif4eb^os84/os84	standard conditions	Fig. 4. from Dockery et al., 2026
whole organism viability, abnormal	eif4e1c^pd370/pd370; eif4eb^os84/os84	standard conditions	Fig. 4. from Dockery et al., 2026
cardiac muscle cell cell division increased occurrence, ameliorated	eif4ea^os81/os81; eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
regenerating fin Ab5-mef2 labeling amount, ameliorated	eif4ea^os81/os81; eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
heart regenerating tissue Ab5-mef2 labeling amount, ameliorated	eif4ea^os81/os81; eif4eb^os83/os83	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell 2-D shape, ameliorated	eif4ea^os81/os81; eif4eb^os83/os83	standard conditions	Fig. 7. from Dockery et al., 2026
heart regenerating tissue Ab5-mef2 labeling amount, ameliorated	eif4ea^os81/os81; eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell 2-D shape, ameliorated	eif4ea^os81/os81; eif4eb^os84/os84	standard conditions	Fig. 7. from Dockery et al., 2026
regenerating fin Ab5-mef2 labeling amount, ameliorated	eif4ea^os81/os81; eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
cardiac muscle cell cell division increased occurrence, ameliorated	eif4ea^os81/os81; eif4eb^os84/os84	amputation: cardiac ventricle	Fig. 6. from Dockery et al., 2026
whole organism viability, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83	standard conditions	Fig. 5. from Dockery et al., 2026
heart Ab5-mef2 labeling decreased distribution, abnormal	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83	standard conditions	Fig. 5. from Dockery et al., 2026
whole organism decreased mass, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83	standard conditions	Fig. 5. from Dockery et al., 2026
whole organism shortened, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83	standard conditions	Fig. 5. from Dockery et al., 2026
cardiac muscle cell decreased amount, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os83/os83	standard conditions	Fig. 5. from Dockery et al., 2026
whole organism viability, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84	standard conditions	Fig. 5. from Dockery et al., 2026
whole organism decreased mass, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84	standard conditions	Fig. 5. from Dockery et al., 2026
heart Ab5-mef2 labeling decreased distribution, abnormal	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84	standard conditions	Fig. 5. from Dockery et al., 2026
whole organism shortened, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84	standard conditions	Fig. 5. from Dockery et al., 2026
cardiac muscle cell decreased amount, exacerbated	eif4e1c^pd370/pd370; eif4ea^os81/+; eif4eb^os84/os84	standard conditions	Fig. 5. from Dockery et al., 2026

Citations