CRISPR

CRISPR1-sv2a

ID

ZDB-CRISPR-221007-1

Name

CRISPR1-sv2a

Previous Names

None

Target

sv2a (1)

Sequence

5' - GTGGACCCTCTACTTTGTGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
lv105	sv2a

Expression

Gene expression in Wild Types + CRISPR1-sv2a

No data available

Phenotype

Phenotype resulting from CRISPR1-sv2a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sv2a

Phenotype	Fish	Conditions	Figures
neuronal action potential propagation process quality, ameliorated	sv2a^lv105/lv105 (AB)	chemical treatment by environment: (5Z)-5-(quinoxalin-6-ylmethylidene)-1,3-thiazolidine-2,4-dione	FIGURE 5 from Zhang et al., 2022
whole organism swimming behavior increased process quality, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
integument increased pigmentation, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
neuronal action potential propagation process quality, ameliorated	sv2a^lv105/lv105 (AB)	chemical treatment by environment: levetiracetam	FIGURE 3 from Zhang et al., 2022
whole organism sv2a expression decreased amount, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 1 from Zhang et al., 2022
post-vent region curved, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
neuronal action potential propagation increased process quality, abnormal	sv2a^lv105/lv105 (AB)	control	FIGURE 3 , FIGURE 5 from Zhang et al., 2022
neuronal action potential propagation process quality, ameliorated	sv2a^lv105/lv105 (AB)	chemical treatment by environment: alvocidib	FIGURE 5 from Zhang et al., 2022
dorsal telencephalon nucleus decreased amount, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 3 from Zhang et al., 2022
whole organism decreased life span, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
neuronal action potential propagation process quality, ameliorated	sv2a^lv105/lv105 (AB)	chemical treatment by environment: sodium valproate	FIGURE 3 from Zhang et al., 2022
whole organism dead, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
neuronal action potential propagation increased process quality, abnormal	sv2a^lv105/lv105 (AB)	chemical treatment by environment: 2-(2-amino-3-methoxyphenyl)chromen-4-one	FIGURE 5 from Zhang et al., 2022
swim bladder uninflated, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
whole organism viability, abnormal	sv2a^lv105/lv105 (AB)	standard conditions	FIGURE 2 from Zhang et al., 2022
whole organism sv2a expression decreased amount, abnormal	sv2a^lv105/+ (AB)	standard conditions	FIGURE 1 from Zhang et al., 2022

Citations