CRISPR

CRISPR2-rgs4

ID

ZDB-CRISPR-220518-1

Name

CRISPR2-rgs4

Previous Names

None

Target

rgs4 (1)

Sequence

5' - GCTTCAAAAGCCAGATCCAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
kbc2	rgs4

Expression

Gene expression in Wild Types + CRISPR2-rgs4

No data available

Phenotype

Phenotype resulting from CRISPR2-rgs4

No data available

Phenotype of all Fish created by or utilizing CRISPR2-rgs4

Phenotype	Fish	Conditions	Figures
spinal cord neuron differentiation decreased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 4 from Mikdache et al., 2021
heart edematous, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 1 from Mikdache et al., 2021
CaP motoneuron axon extension decreased process quality, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 2 from Mikdache et al., 2021
nervous system ab3-akt labeling increased amount, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 5 from Mikdache et al., 2021
CaP motoneuron axon decreased branchiness, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 2 from Mikdache et al., 2021
spinal cord cell population proliferation increased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 4 from Mikdache et al., 2021
whole organism ab1-rps6 labeling decreased amount, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 7 from Mikdache et al., 2021
head decreased size, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 4 from Mikdache et al., 2021
whole organism ab5-akt labeling decreased amount, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 5 from Mikdache et al., 2021
nervous system phosphatidylinositol 3-kinase/protein kinase B signal transduction decreased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 5 from Mikdache et al., 2021
thigmotaxis decreased process quality, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 2 from Mikdache et al., 2021
nervous system ab1-rps6 labeling decreased amount, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 7 from Mikdache et al., 2021
posterior lateral line ganglion has fewer parts of type posterior lateral line ganglion neuron, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 3 from Mikdache et al., 2021
nervous system TOR signaling decreased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 7 from Mikdache et al., 2021
CaP motoneuron axon decreased length, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 2 from Mikdache et al., 2021
whole organism phosphatidylinositol 3-kinase/protein kinase B signal transduction decreased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 5 from Mikdache et al., 2021
whole organism TOR signaling decreased occurrence, abnormal	rgs4^kbc2/kbc2	standard conditions	Figure 7 from Mikdache et al., 2021
spinal cord has fewer parts of type spinal cord neuron, abnormal	rgs4^kbc2/kbc2; knu3Tg	standard conditions	Figure 3 from Mikdache et al., 2021

Citations