CRISPR

CRISPR4-mafbb

ID

ZDB-CRISPR-211228-3

Name

CRISPR4-mafbb

Previous Names

None

Target

mafbb (1)

Sequence

5' - GCGTGCACGTCCCGGGCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3556	mafbb
zf3557	mafbb

Expression

Gene expression in Wild Types + CRISPR4-mafbb

No data available

Phenotype

Phenotype resulting from CRISPR4-mafbb

No data available

Phenotype of all Fish created by or utilizing CRISPR4-mafbb

Phenotype	Fish	Conditions	Figures
whole organism semi-viable, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 1 from Han et al., 2021
basihyal cartilage bent, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
rostral blood island mfap4.1 expression decreased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
scale osteoclast increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 from Han et al., 2021
caudal fin asymmetrical, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 1 from Han et al., 2021
ceratohyal cartilage increased angle to ceratohyal cartilage, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
ceratohyal cartilage bent, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
head nucleate erythrocyte decreased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
yolk spi1b expression decreased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
pharyngeal arch ctsk expression increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 , Figure 6 from Han et al., 2021
post-vent region curved, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 1 from Han et al., 2021
neural arch irregularly shaped, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
hypural 1 morphology, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
hypural fused with hypural, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
post-vent region neutrophil increased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
ventral mandibular arch morphology, ameliorated	mafbb^{zf3556/zf3556} (AB)	chemical treatment by environment: alendronic acid	Figure 6 from Han et al., 2021
post-vent region macrophage increased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
vertebral column curved, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 1 , Figure 3 from Han et al., 2021
ventral mandibular arch protruding, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 1 , Figure 3 , Figure 6 from Han et al., 2021
ceratobranchial 5 tooth bone mineralization decreased process quality, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
hypural decreased volume, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
pharyngeal arch ctsk expression position, ameliorated	mafbb^{zf3556/zf3556} (AB)	chemical treatment by environment: alendronic acid	Figure 6 from Han et al., 2021
brain microglial cell decreased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
head kidney myeloid cell increased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
brain apoeb expression decreased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
pharyngeal arch tnfrsf1b expression increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 from Han et al., 2021
caudal fin osteoclast increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 from Han et al., 2021
trunk ventral region spi1b expression increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
ceratobranchial 5 tooth decreased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
pectoral fin ctsk expression increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 from Han et al., 2021
hemal arch irregularly shaped, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
vertebral column osteoclast increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 2 from Han et al., 2021
Meckel's cartilage bent, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
post-vent region nucleate erythrocyte decreased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
hypural 2 morphology, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 3 from Han et al., 2021
rostral blood island spi1b expression decreased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
head kidney monocyte increased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
post-vent region hematopoietic multipotent progenitor cell decreased amount, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 5 from Han et al., 2021
trunk ventral region mfap4.1 expression increased distribution, abnormal	mafbb^{zf3556/zf3556} (AB)	standard conditions	Figure 4 from Han et al., 2021
whole organism semi-viable, abnormal	mafbb^zf3556/+ (AB)	standard conditions	Figure 1 from Han et al., 2021
scale osteoclast increased distribution, abnormal	mafbb^zf3556/+ (AB)	standard conditions	Figure 2 from Han et al., 2021
pharyngeal arch ctsk expression increased distribution, abnormal	mafbb^zf3556/+ (AB)	standard conditions	Figure 2 , Figure 6 from Han et al., 2021
pharyngeal arch ctsk expression increased distribution, abnormal	mafbb^zf3556/+ (AB)	chemical treatment by environment: alendronic acid	Figure 6 from Han et al., 2021
ventral mandibular arch morphology, ameliorated	mafbb^zf3556/+ (AB)	chemical treatment by environment: alendronic acid	Figure 6 from Han et al., 2021
ventral mandibular arch protruding, abnormal	mafbb^zf3556/+ (AB)	control	Figure 6 from Han et al., 2021
pharyngeal arch tnfrsf1b expression increased distribution, abnormal	mafbb^zf3556/+ (AB)	standard conditions	Figure 2 from Han et al., 2021
pectoral fin ctsk expression increased distribution, abnormal	mafbb^zf3556/+ (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast nfatc1 expression increased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast ocstamp expression increased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast acp5a expression increased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast fosab expression increased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast ctsk expression increased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
osteoclast tnfrsf11b expression decreased amount, abnormal	mafbb^zf3556/+; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
macrophage decreased amount, abnormal	mafbb^{zf3556/zf3556}; gl23Tg (AB)	standard conditions	Figure 4 from Han et al., 2021
hemal arch EGFP expression increased distribution, abnormal	mafbb^{zf3556/zf3556}; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021
pharyngeal arch EGFP expression position, ameliorated	mafbb^{zf3556/zf3556}; zf3555Tg (AB)	chemical treatment by environment: alendronic acid	Figure 6 from Han et al., 2021
pharyngeal arch EGFP expression increased distribution, abnormal	mafbb^{zf3556/zf3556}; zf3555Tg (AB)	control	Figure 6 from Han et al., 2021
neural arch EGFP expression increased distribution, abnormal	mafbb^{zf3556/zf3556}; zf3555Tg (AB)	standard conditions	Figure 2 from Han et al., 2021

Citations