CRISPR

CRISPR1-pln1

ID

ZDB-CRISPR-190304-6

Name

CRISPR1-pln1

Previous Names

CRISPR1-pln
Pln_p.R14Del (1)

Target

pln1 (1)

Sequence

5' - GGCACGGGCGGCCATTCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The second "G" in the sequence does not match the current sequence in Ensembl. The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hu10742	pln1

Expression

Gene expression in Wild Types + CRISPR1-pln1

No data available

Phenotype

Phenotype resulting from CRISPR1-pln1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-pln1

Phenotype	Fish	Conditions	Figures
heart connective tissue replacement involved in inflammatory response wound healing increased occurrence, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
epicardium lipid droplet formation increased occurrence, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 1 from Kamel et al., 2021
epicardium tbx18 expression increased amount, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
heart collagen biosynthetic process increased occurrence, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 1 from Kamel et al., 2021
epicardium tbx18 expression increased distribution, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
cardiac muscle cell action potential decreased duration, abnormal	pln1^{hu10742/hu10742}	primary cell culture: cardiac muscle cell, chemical treatment by environment: Istaroxime	Fig. 7 from Kamel et al., 2021
myocardium myl7 expression decreased amount, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
heart leukocyte grna.2 expression increased amount, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
myocardium myl7 expression decreased distribution, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
epicardium postnb expression increased amount, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
epicardium postnb expression increased distribution, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
heart leukocyte grna.1 expression increased amount, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
heart leukocyte grna.1 expression increased distribution, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
cardiac muscle cell action potential irregular rhythm, ameliorated	pln1^{hu10742/hu10742}	primary cell culture: cardiac muscle cell, chemical treatment by environment: Istaroxime	Fig. 7 from Kamel et al., 2021
heart increased size, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 1 from Kamel et al., 2021
cardiac muscle cell action potential irregular rhythm, abnormal	pln1^{hu10742/hu10742}	primary cell culture: cardiac muscle cell	Fig. 4 from Kamel et al., 2021
heart leukocyte grna.2 expression increased distribution, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 2 from Kamel et al., 2021
regulation of heart contraction irregular rhythm, abnormal	pln1^{hu10742/hu10742}	standard conditions	Fig. 3 from Kamel et al., 2021
cardiac muscle cell regulation of calcium ion transmembrane transport decreased process quality, abnormal	pln1^{hu10742/hu10742}	primary cell culture: cardiac muscle cell	Fig. 5 from Kamel et al., 2021
heart contraction amplitude, abnormal	pln1^{hu10742/hu10742}; ccu1Tg/ccu1Tg; hu6531Tg/hu6531Tg	chemical treatment by environment: Istaroxime	Fig. 6 from Kamel et al., 2021
cardiac muscle cell regulation of calcium ion transmembrane transport decreased process quality, ameliorated	pln1^{hu10742/hu10742}; ccu1Tg/ccu1Tg; hu6531Tg/hu6531Tg	chemical treatment by environment: Istaroxime	Fig. 6 from Kamel et al., 2021
cardiac muscle cell regulation of calcium ion transmembrane transport decreased process quality, abnormal	pln1^{hu10742/hu10742}; ccu1Tg/ccu1Tg; hu6531Tg/hu6531Tg	standard conditions	Fig. 5 , Fig. 6 from Kamel et al., 2021
heart collagen biosynthetic process increased occurrence, exacerbated	pln1^{hu10742/hu10742}; pln2^{zf3815/zf3815}	standard conditions	Fig. Supplemental 4 from Kamel et al., 2021
heart increased size, exacerbated	pln1^{hu10742/hu10742}; pln2^{zf3815/zf3815}	standard conditions	Fig. Supplemental 4 from Kamel et al., 2021
epicardium lipid droplet formation increased occurrence, exacerbated	pln1^{hu10742/hu10742}; pln2^{zf3815/zf3815}	standard conditions	Fig. Supplemental 4 from Kamel et al., 2021

Citations