PUBLICATION
Using retroviruses as a mutagenesis tool to explore the zebrafish genome
- Authors
- Jao, L.E., Maddison, L., Chen, W., and Burgess, S.M.
- ID
- ZDB-PUB-081105-13
- Date
- 2008
- Source
- Briefings in functional genomics & proteomics 7(6): 427-443 (Review)
- Registered Authors
- Burgess, Shawn, Chen, Wenbiao
- Keywords
- genetics, moloney murine leukemia virus, enhancer traps, gene traps, linker-mediated PCR, zebrafish
- MeSH Terms
-
- Animals
- Genome*
- Models, Biological*
- Mutagenesis*
- Retroviridae/genetics*
- Zebrafish/genetics*
- PubMed
- 18977782 Full text @ Brief. Funct. Genomic. Proteomic.
Citation
Jao, L.E., Maddison, L., Chen, W., and Burgess, S.M. (2008) Using retroviruses as a mutagenesis tool to explore the zebrafish genome. Briefings in functional genomics & proteomics. 7(6):427-443.
Abstract
We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping