PUBLICATION
Developmentally Regulated Impediments to Skin Reinnervation by Injured Peripheral Sensory Axon Terminals
- Authors
- O'Brien, G.S., Martin, S.M., Söllner, C., Wright, G.J., Becker, C.G., Portera-Cailliau, C., and Sagasti, A.
- ID
- ZDB-PUB-091215-44
- Date
- 2009
- Source
- Current biology : CB 19(24): 2086-2090 (Journal)
- Registered Authors
- Becker, Catherina G., O'Brien, Georgeann, Sagasti, Alvaro, Söllner, Christian, Wright, Gavin J.
- Keywords
- MOLNEURO
- MeSH Terms
-
- Trigeminal Nerve Injuries
- Nerve Regeneration/physiology*
- Microscopy, Confocal
- Receptors, Cell Surface/genetics
- Receptors, Cell Surface/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish
- Trigeminal Nerve/cytology*
- Neuronal Plasticity/physiology*
- rhoA GTP-Binding Protein/metabolism
- Mutagenesis, Site-Directed
- DNA, Complementary/genetics
- Sensory Receptor Cells/physiology*
- Skin/innervation*
- DNA Primers/genetics
- Presynaptic Terminals/physiology*
- Axotomy
- Animals
- PubMed
- 19962310 Full text @ Curr. Biol.
Citation
O'Brien, G.S., Martin, S.M., Söllner, C., Wright, G.J., Becker, C.G., Portera-Cailliau, C., and Sagasti, A. (2009) Developmentally Regulated Impediments to Skin Reinnervation by Injured Peripheral Sensory Axon Terminals. Current biology : CB. 19(24):2086-2090.
Abstract
The structural plasticity of neurites in the central nervous system (CNS) diminishes dramatically after initial development, but the peripheral nervous system (PNS) retains substantial plasticity into adulthood. Nevertheless, functional reinnervation by injured peripheral sensory neurons is often incomplete [1-6]. To investigate the developmental control of skin reinnervation, we imaged the regeneration of trigeminal sensory axon terminals in live zebrafish larvae following laser axotomy. When axons were injured during early stages of outgrowth, regenerating and uninjured axons grew into denervated skin and competed with one another for territory. At later stages, after the establishment of peripheral arbor territories, the ability of uninjured neighbors to sprout diminished severely, and although injured axons reinitiated growth, they were repelled by denervated skin. Regenerating axons were repelled specifically by their former territories, suggesting that local inhibitory factors persist in these regions. Antagonizing the function of several members of the Nogo receptor (NgR)/RhoA pathway improved the capacity of injured axons to grow into denervated skin. Thus, as in the CNS, impediments to reinnervation in the PNS arise after initial establishment of axon arbor structure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping