PUBLICATION

Mutations affecting the secretory COPII coat component SEC23B cause congenital dyserythropoietic anemia type II

Authors
Schwarz, K., Iolascon, A., Verissimo, F., Trede, N.S., Horsley, W., Chen, W., Paw, B.H., Hopfner, K.P., Holzmann, K., Russo, R., Esposito, M.R., Spano, D., De Falco, L., Heinrich, K., Joggerst, B., Rojewski, M.T., Perrotta, S., Denecke, J., Pannicke, U., Delaunay, J., Pepperkok, R., and Heimpel, H.
ID
ZDB-PUB-090706-6
Date
2009
Source
Nature Genetics   41(8): 936-940 (Journal)
Registered Authors
Paw, Barry, Trede, Nick
Keywords
none
MeSH Terms
  • Anemia, Dyserythropoietic, Congenital/genetics*
  • Animals
  • COP-Coated Vesicles/genetics*
  • Cell Line
  • Cell Nucleus/genetics
  • DNA Mutational Analysis
  • Erythroid Cells/metabolism
  • Humans
  • Jaw/pathology
  • Mutation/genetics*
  • Phenotype
  • Vesicular Transport Proteins/genetics*
  • Zebrafish/genetics
PubMed
19561605 Full text @ Nat. Genet.
Abstract
Congenital dyserythropoietic anemias (CDAs) are phenotypically and genotypically heterogeneous diseases. CDA type II (CDAII) is the most frequent CDA. It is characterized by ineffective erythropoiesis and by the presence of bi- and multinucleated erythroblasts in bone marrow, with nuclei of equal size and DNA content, suggesting a cytokinesis disturbance. Other features of the peripheral red blood cells are protein and lipid dysglycosylation and endoplasmic reticulum double-membrane remnants. Development of other hematopoietic lineages is normal. Individuals with CDAII show progressive splenomegaly, gallstones and iron overload potentially with liver cirrhosis or cardiac failure. Here we show that the gene encoding the secretory COPII component SEC23B is mutated in CDAII. Short hairpin RNA (shRNA)-mediated suppression of SEC23B expression recapitulates the cytokinesis defect. Knockdown of zebrafish sec23b also leads to aberrant erythrocyte development. Our results provide in vivo evidence for SEC23B selectivity in erythroid differentiation and show that SEC23A and SEC23B, although highly related paralogous secretory COPII components, are nonredundant in erythrocyte maturation.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping