The length (L) and width (w) of proximal villi were measured and used to obtain length by width ratios for each villus.

Relative gene expression of the group is represented as a fold change relative to the negative control group (fold change = 1). Values provided are mean fold change + S.E.M (standard error of the mean). Results of One-way ANOVA and LSD test are shown on graphs when significant. Significant differences (p < 0.05) between groups are represented by different letters.

This figure presents two histological samples from the proximal intestines of zebrafish. Fig. A is a sample from the (−) Control group and Fig. B is from the Ala-Glu group. These images were obtained using LAS V4.4 software (Leica Camera, Wetzler, Germany, www.leica-microsystems.com).

The concentration of indispensable free amino acids (μmol/g) in zebrafish muscle. Bars represent level (± std. dev) at 3 h after feeding. Line represents level at 24 h after feeding reflecting the baseline. Results of One-way ANOVA and LSD test are shown on graphs when significant. Significant differences (p < 0.05) between groups are represented by different letters.

The concentration of dispensable free amino acids (μmol/g) in zebrafish muscle. Bars represent level (± std. dev) at 3 h after feeding. Line represents level at 24 h after feeding reflecting the baseline. Results of One-way ANOVA and LSD test are shown on graphs when significant. Significant differences (p < 0.05) between groups are represented by different letters.

Acknowledgments
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Reprinted from Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 257, Molinari, G.S., Wojno, M., McCracken, V.J., Kwasek, K., The use of dipeptide supplementation as a means of mitigating the negative effects of dietary soybean meal on Zebrafish Danio rerio, 110958, Copyright (2021) with permission from Elsevier. Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.